A PCR machine can rise temperature upto 100 0 C but after that it is not able to lower the temperature below70 0 C automatically. Which step of PCR will be hampered first in this faulty machine? Explain why?

1. If the malfunctioning device cannot achieve a temperature below 70 °C, the initial primer annealing step will face impediments.

2. Each primer possesses a distinct annealing temperature, determined by its A, T, G, C composition, typically ranging from 40-60 °C.