In the process of rDNA technology, if two separate restriction enzymes are used to cut vector and donor DNA then which problem will arise in the formation of rDNA or chimeric DNA? Explain.

1. When employing r-DNA technology, the use of distinct restriction enzymes for cutting the vector and donor DNA leads to fragments possessing different sticky ends.

2. These sticky ends generated from the cutting process are not complementary to each other.

3. The non-complementary nature of the sticky ends hinders spontaneous rejoining of the DNA fragments.

4. This method facilitates the subsequent introduction of foreign DNA into the vector with precision during the gene cloning process.