Acche Marks 1. If the malfunctioning device cannot achieve a temperature below 70 °C, the initial primer annealing step will face impediments. 2. Each primer possesses a distinct annealing temperature, determined by its A, T, G, C composition, typically ranging from 40-60 °C.
The ideal cloning vector should possess the following desired characteristics: 1. Enable independent replication through an origin of replication gene. 2. Facilitate seamless transfer into host cells. 3. Incorporate essential control elements such as promoters, operators, and ribosomal binding sites. 4. Integrate marker genes for antibiotic resistance. 5. Include recognition sites for restriction enzymes within … Read more
(i) Bt cotton: Engineered for insect resistance to protect the crop from pests. (ii) Flavr Savr Tomato: Modified to slow down softening during ripening, enhancing shelf life. (iii) Golden Rice: Genetically modified to be rich in vitamin A, addressing nutritional deficiencies. (iv) Holstein Cow: Genetically altered for increased milk productivity.
Mammalian Artificial Chromosome .
Genetically Modified Organisms .
PCR : Polymerase Chain Reaction.
Deoxyribonucleoside triphosphates
Restriction Endonuclease.
YAC : Yeast Artificial chromosome.
Ca++ ions play a crucial role in facilitating the transfer of recombinant vectors into bacterial host cells.